Random chimeragenesis on transient templates

Random chimeragenesis on transient templates (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate.^[1] For example, RACHITT can be used to generate increased rate and extent of biodesulfurization of diesel by modification of dibenzothiophene mono-oxygenase. DNA shuffling is a similar but less powerful method used in directed evolution experiments.

References

^ Coco, Wayne M.; Levinson, William E.; Crist, Michael J.; Hektor, Harm J.; Darzins, Aldis; Pienkos, Philip T.; Squires, Charles H.; Monticello, Daniel J. (31 March 2001). "DNA shuffling method for generating highly recombined genes and evolved enzymes". Nature Biotechnology. 19 (4): 354–359. doi:10.1038/86744. PMID 11283594. S2CID 35360374.

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[1] Coco, Wayne M.; Levinson, William E.; Crist, Michael J.; Hektor, Harm J.; Darzins, Aldis; Pienkos, Philip T.; Squires, Charles H.; Monticello, Daniel J. (31 March 2001). "DNA shuffling method for generating highly recombined genes and evolved enzymes". Nature Biotechnology. 19 (4): 354–359. doi:10.1038/86744. PMID 11283594. S2CID 35360374.

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